Separation of Spermatozoa with a Combination of Pinched Flow Fractionation and Tangential Filtration
نویسندگان
چکیده
We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other particles. The new design circumvents this effect.
منابع مشابه
Pinched flow fractionation: continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel.
A concept of "pinched flow fractionation" for the continuous size separation and analysis of particles in microfabricated devices has been proposed and demonstrated. In this method, particles suspended in liquid were continuously introduced into a microchannel having a pinched segment and were aligned to one sidewall in the pinched segment by another liquid flow without particles. The particles...
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In a recent paper Yamada et al. [1] propose the novel concept of ”pinched flow fractionation” (PFF) for the continuous size separation and analysis of particles in microfabricated lab-on-a-chip devices. In their description of the basic principle they claim that especially the width of the pinched and broadened segments will affect the separation performance. In the following we comment on the ...
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